This is a slight modification of the method given by Myers and Fine, which, in turn, is a modification of the colorimetric method of Folin and Farmer.1 The only difference in technic is that of adding hydrogen peroxid to hasten oxidation, which considerably shortens the time of making the test. In the method of Myers and Fine, fully fifteen to twenty minutes is required to complete the determination. As here described, the estimation may be completed in from five to ten minutes.
In the method as described by Myers,2 for the determination an amount of urine sufficient to contain between 0.35 and 0.75 mg. of nitrogen is required. This is usually obtained by a 1:25 dilution of urine, although sometimes a dilution of 1:10 is sufficient, as indicated by a low specific gravity. One
c.c. of urine is taken with an Oswald-Folin pipet and diluted to 25 c.c.
Gradwohl RBH, Blaivas AJ. A SIMPLE METHOD FOR THE DETERMINATION OF NITROGEN IN URINE. JAMA. 1916;LXVII(11):809. doi:10.1001/jama.1916.25910110002010a
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