The method described here is a modification of that described by Fonio. Reagents used in the technic are as follows :
Fourteen per cent magnesium sulphate solution. Dissolve 14 Gm. of chemically pure magnesium sulphate in enough distilled water to make a total of 100 cc. Filter through filter paper.
Methyl alcohol. Uniformly good success has been obtained with the methyl alcohol furnished by the National Aniline Company, and other brands now on the market have failed to give equal success. It is for that reason that I specify the use of National Aniline Company methyl alcohol. The alcohol used in fixing blood platelet smears should be labeled "for blood platelet counting." It must not be used for fixing blood films.
Giemsa stain. I recommend the Giemsa stain that the Gradwohl Laboratories have prepared for this purpose, following Professor Giemsa's original formula.1
Neutral distilled water. For the testing of neutral distilled
Gradwohl RBH. A NEW METHOD OF STAINING BLOOD PLATELETS. JAMA. 1935;105(13):1030–1031. doi:10.1001/jama.1935.92760390001008
Customize your JAMA Network experience by selecting one or more topics from the list below.