In 1913 Ito, working with Carl Bruck at Neisser's clinic, devised a specific intradermal test for chancroid —Ducrey bacillus infection. The diagnostic value of this test was later confirmed by Reenstierna, working with a serum containing antibodies of the Ducrey bacillus. Today this test is spoken of as the Ito-Reenstierna reaction.
Ito, for his cultures, used 24 hours old streptobacillus colonies from two Besançon blood agar tubes. The bacilli are suspended in physiologic solution of sodium chloride with the addition of 0.5 per cent phenol and heated at 60 C. for two hours. Reenstierna employed unheated material in the presence of phenol for fourteen days in the icebox. Frei1 in his survey of the entire subject up to 1927 says that he employed the water of condensation. He also states that it makes little difference what method of preparation is employed.
The antigen is employed as an intradermal test,
COLE HN, LEVIN EA. THE INTRADERMAL REACTION FOR CHANCROIDS WITH CHANCROIDAL BUBO PUS. JAMA. 1935;105(25):2040–2044. doi:10.1001/jama.1935.02760510012003
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