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The isolation, in recent years, of minute amounts of substances of profound biologic importance has created a need for various types of microchemical methods. The present monograph, one of a series of monographs on microchemical methods, describes several such methods performed with the aid of the microscope. The first section, by Dr. Ludwig Kofler, describes a reliable method for determining the melting point of less than a millionth of a gram of material. The second section, by the same author, deals with microsublimation and describes the preparation of crystals suitable for determinations of melting points and optical properties by subliming minute amounts of material on a microscopic slide. In both sections the author stresses the fact that these microscopic methods yield information which the macro methods are incapable of giving. In a third section Dr. Adelheid Kofler discusses some optical properties of crystals and describes some simple measurements of crystals,
Mikroskopische Methoden in der Mikrochemie. JAMA. 1936;106(11):948. doi:10.1001/jama.1936.02770110064037
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