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This is a critical and lucid exposition of the chemistry of tissues as studied in microscopic sections. The first part takes up the general methods and limitations. In general, substances in solution in cells cannot be localized because they diffuse during analysis, and substances not in solution may be extracted by preliminary treatment. Therefore the most general method is freezing the tissue. After it is once frozen there are two main methods of treatment: It may be dried without thawing. If paraffin-soluble substances are not to be analyzed, it may be impregnated with paraffin in the dry state and cut into sections and mounted on slides, and if only inorganic substances are to be studied the slide may be heated in a muffle furnace so as to ash the section and then the ash analyzed by various methods. Another method is to cut frozen sections and place them on slides
Histochimie animale: Méthodes et problèmes. JAMA. 1936;107(1):68–69. doi:10.1001/jama.1936.02770270070033
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