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May 5, 1962


JAMA. 1962;180(5):405-406. doi:10.1001/jama.1962.03050180051014

In order to investigate the influence of aldosterone on the transport of sodium by the nephron, Crabbé1 studied isolated toad bladders. Two sets of animals were used, one maintained in distilled water (water toads) and one maintained in a physiologic saline solution (saline toads). After pithing the toads, bladder preparations were made and bathed in Ringer's solution that contained varying concentrations of aldosterone. Such a membrane can move sodium actively from its mucosal to its serosal surface and this process can be measured by the short-circuit current technique. By this means the author showed that active sodium transport can be stimulated by aldosterone in vitro at room temperature after a latent period of about one hour. Concentrations of hormone of less than 7 times those found in the blood of water toads elicited a significant elevation of the short-circuit current.

Stimulation of sodium transport by aldosterone was not associated