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Clinical Notes
January 4, 1965

Effect of Salt Restriction on Experimental Nephrosis

Author Affiliations

From the Department of Medicine, Duke University School of Medicine, Durham, NC.

JAMA. 1965;191(1):51. doi:10.1001/jama.1965.03080010057023

AN EFFICIENT and dependable method to produce a nephrotic syndrome experimentally has been developed, in which young rats are subcutaneously injected with aminonucleoside of puromycin. The animals have proteinuria, hypercholesterolemia, and edema, largely as free fluid in the peritoneal cavity and as gel in the skin. The disease can easily be made so severe that most of the animals die within one to three weeks after the injections.1-3

Steroids did not modify the disease once it had appeared nor did they prevent it from appearing.4 Adenine (6-aminopurine), but not adenosine, temporarily delayed the onset of proteinuria, but by the second week the nephrotic syndrome was fully developed.5

Our report deals with the death rate in this experimental nephrosis in the presence and absence of sodium chloride.

In a typical experiment which may serve as an example, two groups of twenty rats each were used; they were female