In Reply Even though rapid DFA testing is not the reference standard test for diagnosing HSV, it was the best option among those given in the answer choices, and the most rapid diagnostic test available because PCR was not an option at the hospital during the weekend when the infant presented.
Given that PCR is the preferred diagnostic method based on current recommendations,1,2 it was ordered during the week. Meanwhile, the rapid DFA test was sent due to the availability of the test. This case highlights the utility of additional testing methods for diagnosis of neonatal HSV.
Barry Ladizinski, Kachiu C. Lee. Diagnosis of Neonatal Infection With Herpes Simplex Virus—Reply. JAMA. 2014;311(5):528. doi:10.1001/jama.2013.285417