In Reply: In response to Dr Buhimschi and colleagues,
we would like to clarify several points. As we noted in the article, our SELDI-TOF
proteomic profiling was a low-resolution approach to distinguish patients
with and without IAI. The SELDI-TOF mass spectrometry we used (pBluescript
II) did not have the necessary mass accuracy to identify potential proteins.
The 3- to 5-kDa cluster we identified in IAI contained several peaks and each
peak could represent multiple proteins, including defensins. Heine et al1 described defensins in vaginal secretions by using
enzyme-linked immunosorbent assay.
Gravett MG, Nagalla SR. Proteomic Profiling and Intra-amniotic Infection—Reply. JAMA. 2004;292(19):2339–2340. doi:10.1001/jama.292.19.2338-b