The serum diagnosis of syphilis as given to the world by Wassermann, Neisser and Brück1 in 1906, has now become a well-established diagnostic method, generally used in all medical centers, and has proved of value in all fields of medicine.
The Wassermann method is an adaptation of the Bordet-Gengou phenomenon of complement deviation, in which the hemolytic system consists of a suspension of washed red blood cells of the sheep, hemolytic amboceptor made by immunizing rabbits to sheep's red blood cells, and as complement fresh guinea-pig serum. For syphilitic antibody the serum from suspected patients is used, and for antigen a watery extract of a liver of congenital syphilis known to be rich in Spirochæta pallida.
It has been pointed out that there is one factor of error in this method due to the fact that there is in human serum a variable amount of natural antisheep amboceptor which
BULSON AE. THE NOGUCHI SERUM REACTION FOR SYPHILIS AS AN AID TO DIAGNOIS IN EYE LESIONS. JAMA. 1910;55(3):181–186. doi:10.1001/jama.1910.04330030003002
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