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The following notes are published as a caution to those who make routine examinations of the urine for indican. It is sometimes necessary to preserve the urine for a time before the test can be made, and for this purpose formaldehyd solution should not be used; at least not when bleaching powder or potassium permanganate is employed as the oxidizing reagent. I have been unable to find note of this in the texts at my command (Emerson, Wood, Simon), nor have I seen attention called to the fact in any published article. Emerson says, however, "A person must be wary in a chemical examination of such a urine" (i. e., one preserved with formaldehyd solutions).
The tests used are the ordinary ones, as follows: Ten c.c. of concentrated hydrochloric acid is poured into a test-tube containing a like amount of the urine to be tested. This is mixed and potassium
DAVENPORT HI. NOTES ON CERTAIN TESTS FOR INDICAN IN THE URINE. JAMA. 1910;55(10):855–856. doi:10.1001/jama.1910.04330100041013
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