Having successfully applied Harrison's method of tissue growing in vitro to the cultivation of the virus of vaccinia,1 we have here tried the same method with the Spirochaeta pallida. In the present experiment the organisms used were from a culture obtained from Dr. Noguchi. He has kept it growing for some length of time and states that it has lost most of its virulence.
Hanging-drop preparations were made of small pieces of the testes of the rabbits inoculated with spirochetes, to which rabbit plasma was added. The preparations were incubated at 37 C. (98.0 F.). Before incubation preparations examined in the dark field did not show spirochetes nor did those examined during the first few days of incubation. Evidently very few spirochetes were inoculated. The cells of the testes, however, showed
STEINHARDT E. A PRELIMINARY NOTE ON SPIROCHAETA PALLIDA AND LIVING TTISSUE-CELLS IN VITRO. JAMA. 1913;61(20):1810. doi:10.1001/jama.1913.04350210050017
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