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February 16, 1918


Author Affiliations

Chief of Board of Health Laboratory, ANCON, C. Z.

JAMA. 1918;70(7):454. doi:10.1001/jama.1918.26010070004009a

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With the assistance of Mr. J. E. Jacob, I have shown that a 10 per cent. solution of crystalline sodium sulphite can be heated for twenty minutes at 15 pounds pressure with practically no change, and that the 10 per cent. sodium sulphite solution covered with a layer of liquid petrolatum about 1 cm. thick and sterilized in the autoclave can be kept at room temperature for three weeks and probably much longer with but very slight change.

Such a sterilized stock solution of sodium sulphite might render the preparation of Endo agar a somewhat simpler procedure in field hospitals and base hospitals. If the liquid petrolatum carried over into the agar with the pipet is considered a disturbing element, more than the amount of the sulphite to be used should be pipetted into a sterile vessel, and the necessary portion of this should be immediately transferred to the agar with

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