As a result of the observation that a boiling alkaline hypochlorite solution would not destroy the precipitable substance of bacteria, we developed a rapid method for the preparation of a concentrated precipitin antigen from bacteria.1 The same method was found to be applicable to the preparation of a precipitin antigen from pneumonia sputum. In an appreciable proportion of specimens the type of pneumococcus present could be identified by this method in from one half to one hour after the receipt of the sputum. More recently, Mitchell and Muns2 have reported a less complicated method for the preparation of an antigen from sputum. Their reports, as well as the results of our attempts to shorten the time in carrying out the mouse test according to the method of Blake,3 indicated that many sputums are comparatively rich in soluble antigen. This being so, the solution of the pneumococci in
KRUMWIEDE C, VALENTINE E. DETERMINATION OF THE TYPE OF PNEUMOCOCCUS IN THE SPUTUM OF LOBAR PNEUMONIA: A RAPID, SIMPLE METHOD. JAMA. 1918;70(8):513–514. doi:https://doi.org/10.1001/jama.1918.02600080015005
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