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CLINICAL OBSERVATIONS ON THE BACTERIOLOGY OF STREPTOCOCCUS EMPYEMA
The pleural exudates from the cases at Camp Lee were examined both in smears and plate cultures prepared with plain agar to which 10 per cent. of defibrinated human blood had been added.In the fluids obtained by aspiration, hemolytic streptococci alone were found. These were often extremely abundant, a single loop of the exudate yielding from 100,000 to 200,000 colonies in plate cultures.Stained smears from these exudates showed that the majority of the leukocytes present had undergone necrosis and were in process of disintegration. In many cases there was no evidence of phagocytosis. In other, but fewer, cases, phagocytosis was very marked; as many as forty cocci, sometimes in threads of considerable length, more often appearing as diplococci, were seen in a single phagocyte. But there were always more free organisms than the number incorporated, and the phagocytosis never appeared
DUNHAM EK, GRAHAM EA, MITCHELL JF, et al. CASES OF EMPYEMA AT CAMP LEE, VA. PRELIMINARY REPORT. JAMA. 1918;71(6):443–448. doi:https://doi.org/10.1001/jama.1918.26020320003010
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