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A somewhat extensive series of articles have appeared relative to the preservation of complement, and many ingeniously worked out methods have been advocated. It is not the intention now to discuss the merits or demerits of other methods but to offer a method, simple, inexpensive and reliable, which I have used.
I have found that complement from freshly bled guineapig blood, centrifuged, is not so powerful as that which stands over night in the refrigerator and is permitted to separate from the slowly contracting clot. This has repeatedly been observed and corroborated in an extensive series of experiments covering a long period of time, by titrating the same complement shortly after obtaining the blood, and likewise the following morning, using the same amboceptor and sheep corpuscles.
For the preservation of complement, the worker needs a vacuum bottle into which finely cracked ice is dropped until the bottle is filled almost
Moledezky MD. PRESERVATION OF COMPLEMENT. JAMA. 1918;71(12):968–969. doi:https://doi.org/10.1001/jama.1918.26020380004002e
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