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Article
September 28, 1918

A FILTRABLE TOXIC PRODUCT OF THE HEMOLYTIC STREPTOCOCCUS

Author Affiliations

First Lieutenant, M. R. C., U. S. Army; Contract Surgeon, U. S. Army BALTIMORE

From the Pathological Laboratory of the Johns Hopkins University.

JAMA. 1918;71(13):1048-1049. doi:10.1001/jama.1918.02600390032010

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Abstract

The following is a brief statement of the methods and results of experiments in which a filtrable substance toxic for rabbits has been obtained from cultures of hemolytic streptococci isolated in cases of bronchopneumonia occurring in Army camps. Though various workers at different times have obtained toxic materials from streptococci, yet we have so far been unable to find any report of methods or results directly analogous to these. The observations that led to the work were made simultaneously and independently by each of us.

The essential feature of the method was the cultivation of the streptococcus in a medium consisting of sterile, defibrinated rabbit's blood diluted with Locke's solution. The Berkefeld filtrates from such cultures were acutely toxic for rabbits, while uninoculated hemolyzed controls incubated for the same time were nontoxic. Toxicity in the culture medium did not appreciably appear before eighteen hours, reached its maximum at about forty-eight

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