Most of the material on which these studies are based came from the peridental membrane clinic at the Northwestern University Dental School. In all of these, therefore, there are alterations of a high degree. At the same time, there are portions of each fragment that are only moderately changed. Zenker's fluid was used most often for fixation, and either celloidin or paraffin used for embedding. Paraffin was finally selected as the routine procedure because it was possible to get thinner sections and serial sections were more readily obtained. Hematoxylin and eosin, or methylene blue and eosin were the chief stains used. Occasionally methylene blue was used alone. The serial section procedure has more than paid for the trouble it entails, as it is only in this manner that the changes can be followed straight through the block, and reconstructions of the various parts achieved. An especially good illustration of this
HATTON EH. MICROSCOPIC STUDIES OF DISEASED PERIDENTAL TISSUES. JAMA. 1918;71(19):1549–1552. doi:https://doi.org/10.1001/jama.1918.02600450025007
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