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November 23, 1918


Author Affiliations

Fort Lyon, Colo.

JAMA. 1918;71(21):1766. doi:10.1001/jama.1918.02600470064029

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To the Editor:  —Being dependent on a rather uncertain source for distilled water suitable for use with Wright's or Jenner's blood stains, I have finally found a method with which both "power house" distilled water and tap water may be used with success. Neither waters are filtered before use.Webster's "Diagnostic Methods," Edition 5, and Simon's "Clinical Diagnosis," Edition 9, give different methods of preparing and using Pappenheim's panoptic stain, and by combining parts of each formula and method I have hit on a stain that is quick, clean and reliable (so far) for blood.From Simon I make up Giemsa stain thus:Dyes are ground up in the alcohol and added to the glycerin. To 150 c.c. of the foregoing solution I add 100 c.c. of Jenner's stain (1.5:1) and use thus:I fix blood smears by immersion in this stain in a Coplin staining jar for one minute,

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