The method for the determination of the hemoglobin content of the blood introduced by Sahli is probably one of the most commonly used today. The method is fairly reliable and the apparatus required is not complicated; it consists of a simple color standard (usually acid hematin) and a calibrated tube in which the measured amount of blood is treated appropriately for comparison. Clinical chemists have recognized, however, that the Sahli method is open to certain objections: the inaccuracies due to the variations in the calibrations and in the construction of the tubes; the fading of the standard;1 the variations of the readings in different lights; the delay in the development of the permanent color, and the "personal equation."
About two years ago, Palmer2 suggested converting the hemoglobin to carbon monoxid-hemoglobin and comparing this with a standard solution of like composition. The comparisons were made in a colorimeter, a
HEMOGLOBIN DETERMINATION. JAMA. 1919;73(26):1942. doi:10.1001/jama.1919.02610520032020
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