We wish to report briefly the results of some experiments on the specific precipitinogenic action of leukocytes. The first experiments were made with extracts of dog and guinea-pig leukocytes obtained from the injection of suspension of wheat gluten in the pleural or peritoneal cavity. The leukocytes were washed many times in salt solution, and definite quantities by weight suspended in water and shaken thoroughly; an equal amount of physiologic sodium chlorid solution of double strength was then added, and the strength of the extract designated by the relation of the weight of the leukocytes extracted to the total quantity of the fluid used, this being usually 1: 10 or 20 or 100. Rabbits were injected intravenously at three-day intervals with increasing quantities of such extracts, say 2, 3, 4, 5 and 6 c.c. of a 1: 20 extract, and the serum was tested about the fifth day after the last
HEKTOEN L, MENNE FR. SPECIFIC PRECIPITIN REACTION OF LEUKOCYTES. JAMA. 1922;79(16):1328. doi:10.1001/jama.1922.02640160048015
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