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November 4, 1922


JAMA. 1922;79(19):1611-1612. doi:10.1001/jama.1922.02640190049021

The extravital cultivation of tissue cells is a comparatively new accomplishment of the biologic sciences. Scarcely a dozen years have elapsed since Harrison1 published the outcome of his pioneer experiments on the ameboid outgrowth of fibers from isolated nerve cells in vitro. This has not remained an isolated accomplishment. Connective tissue cells and epithelium also have shown a capacity for uninterrupted reproduction in isolated masses, notably in the hands of Carrel and his associates at the Rockefeller Institute, provided the environmental conditions are made favorable for the microscopic structures under investigation. The technic of such studies involves experimentation of a high degree of refinement, in which asepsis is an added requisite. Heretofore, one essential of the method of obtaining a pure strain of any type of tissue cell has consisted in the isolation of tissues for cultivation from portions of the organism where they may be obtained free from

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