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In the routine examination of prostatic secretion for the presence or absence of bacteria, and particularly gonococci, it is of the greatest importance to examine the cytoplasm of large numbers of pus cells. The small amount of pus that may be present is often so diluted with prostatic secretion that direct smears of the material are so thin as to make the finding of any number of pus cells a difficult task. The thicker parts of films dry slowly with consequent shrinking of the cytoplasm to a mere band surrounding the nucleus, within which it is almost impossible to recognize the morphologic appearance of organisms that may be present. The normal fluid itself also stains, making the examination of very thick areas impossible. After many attempts to devise a practical method of concentration of the cellular elements in prostatic secretion, the technic here described, which consists essentially of the use
JOHNS FM. A METHOD FOR THE CONCENTRATION OF CELLS AND BACTERIA IN PROSTATIC SECRETION. JAMA. 1923;80(7):463–464. doi:10.1001/jama.1923.02640340019008
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