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April 14, 1923


Author Affiliations

Maui, Hawaii

JAMA. 1923;80(15):1092. doi:10.1001/jama.1923.02640420054031

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To the Editor:  —At the Kula Sanitarium, for some time, we have been using, as a routine, a method of staining tubercle bacilli which is eminently satisfactory and is superior to the usual Ziehl-Neelsen stain.A smear of sputum is prepared and fixed in the usual manner. It is steamed for two full minutes with anilin gentian violet prepared according to the method of Weigert, as described by Stitt, boiling being avoided. The slide is rinsed with running water and covered with gram-iodin solution (Lugol) for two minutes. It is decolorized with acid alcohol (usual solution), washed in running water, and counterstained with 0.1 per cent, solution of pyronin for several seconds. Though we have found pyronin to be the best counterstain, any other may be used.In the medical literature accessible to me I have not been able to find this method described and would appreciate greatly if you

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