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In the United States, the use of the Neubauer ruling shown in Figure 1 has been rather general for hemacytometer chambers. Professors in medical colleges, however, have had more or less difficulty in teaching the student to make the correct count because of the extra lines, which were originally added for the purpose of making the principal subdivisions of the central square millimeter area more readily discernible. Actually, these extra lines have led to confusion, resulting in erroneous counts, and the Neubauer ruling has not, therefore, been an unqualified success.
A new type of ruling, shown in Figure 2, a modification of the Neubauer, was recently submitted to the Bureau of Standards for test. This new ruling clearly defines the boundaries of the major subdivisions, and it would appear to possess an advantage to the physician using the instrument in the diagnosis of disease, because of the greater certainty with
Judson LV. NEW RULING FOR HEMACYTOMETER CHAMBERS. JAMA. 1925;84(13):947. doi:10.1001/jama.1925.26620390001005a
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