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Article
June 6, 1925

RAPID PNEUMOCOCCUS TYPE DETERMINATION IN LOBAR PNEUMONIA BY KRUMWIEDE'S METHOD

Author Affiliations

Boston
From the medical clinic of the Peter Bent Brigham Hospital.

JAMA. 1925;84(23):1733-1734. doi:10.1001/jama.1925.26620490001011
Abstract

The serum treatment of lobar pneumonia, to be successful, depends in part on early diagnosis of the type of pneumococcus responsible for the infection. The mouse method rarely yields a result before ten hours or more. In the effort to shorten this time, Krumwiede,1 Oliver,2 and Mitchell and Muns3 have suggested various methods of direct precipitin determination on sputum without preliminary enrichment in the mouse peritoneum. Krumwiede's method is the simplest of these. The procedure he described consisted in coagulating from 3 to 10 c.c. of sputum in a boiling water-bath, breaking up the clot, adding salt solution and extracting soluble antigen in the water-bath, and layering the supernatant fluid obtained by centrifugalizing on type serums. Of twenty-five tests in which the result was checked by the mouse method, nineteen gave fixed types of pneumococci in the mouse, and of these fifteen gave a correct type reaction.

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