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September 15, 1923

A NOTE ON THE PURIFICATION AND ADMINISTRATION OF INSULIN

JAMA. 1923;81(11):920-921. doi:10.1001/jama.1923.02650110050013

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Abstract

In view of the work recently reported by Mills on absorption of insulin from the intestine, and the work by Best and Scott on the recovery of insulin from urine, it was thought best to report a summary of the results obtained with insulin in a similar type of experiments during the past year. The details of the work will be left for a later communication.

The Doisy-Somgyri-Shafer method of preparing insulin was modified and used with great success. One of the modifications was to precipitate with one volume of 95 per cent. alcohol, whereas the workers mentioned used nine volumes to precipitate the final product. This precipitate, thrown down by one volume of 95 per cent, alcohol, raised the blood sugar of animals when injected subcutaneously. When a small amount was given intraperitoneally to rats or guinea-pigs, death invariably resulted within fifteen minutes. The injection of this substance subcutaneously

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