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Article
June 20, 1925

Technique of Tissue Culture "In Vitro."

JAMA. 1925;84(25):1943. doi:10.1001/jama.1925.02660510059032

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Abstract

Tissue Culture in Relation to Growth and Differentiation. By T. S. P. Strangeways, Lecturer in Special Pathology in the University of Cambridge. Cloth. Price, 5 shillings net. Pp. 50, with 4 illustrations. Cambridge: W. Heffer & Sons, Ltd., 1924.

In the first book, Strangeways describes with many details the complex technics that the student must master before he can cultivate tissues in vitro successfully. Minute instructions are given for the setting up of the apparatus, its sterilization, the preparation of the medium for the tissues, and of the cultures themselves. Methods for staining cultures, both before and after fixation, are described. The book will doubtless be of great help and permanent value for the beginner. At the same time, it cannot lead the student to the newer procedures that have been developed during the last few years in this country, for the isolation and maintenance of pure strains of cells,

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