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August 1, 1925


JAMA. 1925;85(5):371. doi:10.1001/jama.1925.02670050055026

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Detection of Spirochaeta Pallida  Dr. A. Aleixo, a professor in the Bello Horizonete Medical School, has studied the detection of Spirochaeta pallida by puncture of the chancre. The area about the chancre is sterilized and local anesthesia is used. A needle is inserted almost parallel to the surface, so as to cover practically the whole diameter of the chancre. With a Luer syringe, fluid is aspirated slowly. The fluid obtained in this way from a chancre usually contains few cells but many spirochetes. Tribondeau-Fontana's method is used for staining smears. Dr. Aleixo has applied this procedure in fifty proved or suspected cases; of these, forty-three, or 91.4 per cent., proved positive. This group included five cases of healed chancre, one case of phimosis, with a hidden chancre, and four cases in which identification of the organism proved difficult. Large chancres do not always yield the greatest number of spirochetes. Among

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