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Article
December 5, 1925

A METHOD FOR WASHING FIXED TISSUE

Author Affiliations

Pittsburgh
From the Bureau of Mines, Department of Commerce.

JAMA. 1925;85(23):1811. doi:10.1001/jama.1925.26710230001012

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Abstract

In the preparation of good histologic sections for microscopic examination, it is essential that all tissue fixed in Zenker's solution or other fixatives containing bichromate should be thoroughly washed in water.

None of the methods used were found to be entirely satisfactory, and it occurred to me that a tea ball might answer the purpose. It fulfilled the requirements and has been found to be very convenient. The tea ball is of the conventional aluminum type that screws together and has a chain with a hook fastened to the top. The ball containing the tissue is simply placed in a vessel of running water, with the hook fastened on the side. With this device, the specimens can be thoroughly washed without injury. The identification tag can either be placed with the tissue or be fastened on the chain. The ball is cheap and easily cleaned.

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