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To the Editor:
—Dr. Kapsinow, in his article on a new test for bile pigments in urine, bile and blood serum (The Journal, March 1, p. 687) reports that the Obermayer indican test is also a bile test, being more immediate and more delicate than the Gmelin and Rosenbach reactions. In searching for a new bile test, I finally succeeded in finding that if 5 c.c. of concentrated hydrochloric acid is added to an equal amount of urine in a test tube, and the test tube is slowly inverted several times, a drop of hydrogen peroxid then being allowed to flow down the side of the tube, in the presence of bile a deep green ring appears between the peroxid and the mixture below. The area above the ring assumes a dirty green discoloration. As the tube is allowed to stand, the dirty green discoloration gradually descends, so that in
Gould WL. NEW TEST FOR BILE PIGMENTS. JAMA. 1924;82(12):990. doi:10.1001/jama.1924.02650380058032
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