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April 19, 1924


Author Affiliations

San Joaquin, Calif.

JAMA. 1924;82(16):1285. doi:10.1001/jama.1924.02650420049032

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To the Editor:  —I have on several occasions used the albuminous portion of a hard boiled egg as an emergency culture medium. As none of the laboratory men to whom I have mentioned it had ever heard of its use, I decided to report it. I prepare the egg by boiling for five minutes; then I drop it in cold water for two or three minutes, split the egg, shell and all, lengthwise, remove the yolk from each half and make the culture on the concave surface, and then put the two halves together and wrap them up ready to send to the laboratory. The culture will keep moist and remain warm for a considerable time.I first used this in a case of suspected diphtheria that was 30 miles from the nearest prepared culture tube, and the report came back so good that I have used it several times

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