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Article
June 7, 1924

DEFECTS IN TEST TUBES AS A CAUSE OF ANOMALOUS REACTIONS

Author Affiliations

South Bend, Ind.

JAMA. 1924;82(23):1863. doi:10.1001/jama.1924.26520490003012b

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Abstract

Most workers in serology or allied subjects in which tests are made in glass tubes and set aside for several hours for reactions to occur have probably obtained results at variance with expectations or, if the tests were done in duplicate, results showing marked disagreement. I refer to such experiences as these: In a set of colloidal gold tubes all showing no change, one shows a change of 5. In washing erythrocytes in duplicate tubes, the longer the washing the more hemolysis appears in one tube, while the cells in the other tube wash clean. In a set of Wassermann tests done with several antigens, a serum showing a negative test with a cholesterolized antigen showed a four plus reaction with a plain extract antigen. The tubes in all these instances had been cleaned with the standard sulphuric acid and sodium bichromate mixture over night, and rinsed with running tap

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