In June, 1925, we1 reported the presence of a toxic substance in the Berkefeld V filtrate of broth cultures of Streptococcus hemolyticus isolated from the blood of patients with puerperal fever. Intracutaneous injections of the filtrates (sterile) produced inflammatory reactions, which varied in degree and incidence with the dilution of the filtrate and the presumable immunity of the patient. There was a definite latent period between injection and reaction.
The present report concerns experiments with the toxic substance in the filtrate, which were carried out in order to determine further characteristics of a toxin and the specificity of the organism. Strain A of our puerperal streptococci and the Berkefeld V filtrate of its cultures were used exclusively in the study.
HEAT LABILE PROPERTY
One cubic centimeter of the 1: 100 dilution of the filtrate was heated in a water bath at 70 C. for fifteen minutes and a similar
LASH AF, KAPLAN B. PUERPERAL FEVER: II. STREPTOCOCCUS HEMOLYTICUS TOXIN AND ANTITOXIN. JAMA. 1926;86(16):1197–1198. doi:10.1001/jama.1926.02670420021005
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