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Article
April 8, 1933

THE USE OF AUTOLYZED LIVER IN THE TREATMENT OF PERNICIOUS ANEMIA: A PRELIMINARY CLINICAL REPORT

Author Affiliations

PITTSBURGH
From the Departments of Medicine and Physiological Chemistry, University of Pittsburgh School of Medicine.

JAMA. 1933;100(14):1084-1086. doi:10.1001/jama.1933.02740140008002
Abstract

In a previous communication we1 reported the results of several observations, suggesting that autolysis markedly increases the potency of liver in the treatment of pernicious anemia. Further work has substantiated the preliminary observations, and, because of their important relation to practical liver therapy and theories regarding the possible nature of the beneficial agent, the results of treatment of thirteen cases of pernicious anemia with autolyzed liver are here reported.

PREPARATION AND METHOD OF ADMINISTRATION  Fresh beef liver obtained from the slaughter house was ground and fiftieth-normal hydrochloric acid was added in the proportion of 1,000 Gm. of minced liver to 5,000 cc. of fiftieth-normal hydrochloric acid. Twenty-five cubic centimeters of chloroform per kilogram of liver was added as a preservative. The mixture was thoroughly shaken, placed in an incubator at 37 C. and allowed to undergo autolysis for an average period of ten days. It was shaken daily during

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