A biologic method has been devised for identifying a specific skin-reacting substance in the blood serum of tuberculous patients and animals. The point of departure was an earlier observation, reported elsewhere, 1 that sensitization in normal guinea-pigs could be accomplished with fractional tuberculins prepared from nonprotein substrates.
Duplicate series of healthy white guinea-pigs, weighing from 450 to 500 Gm., were used in each set of experiments. These were grouped as follows: (1) normal guinea-pigs previously injected with tuberculin fractions; (2) normal, untreated guinea-pigs, and (3) tuberculous guinea-pigs with early, moderately advanced and far advanced infections.
The animals were tested with (a) human serums from early and far advanced cases of tuberculosis; (b) the same serums heated at from 60 to 65 C. on a water bath for forty-five minutes; (c) normal human serum, and (d) guinea-pig serum from early and moribund stages of tuberculosis. Intracutaneous injections of 0.02 cc. of
EBERSON F. STUDIES IN TUBERCULOSIS: VIII. IDENTIFICATION OF A SKIN-REACTING SUBSTANCE IN BLOOD SERUMS FROM TUBERCULOUS PATIENTS AND ANIMALS. JAMA. 1927;88(5):313–315. doi:10.1001/jama.1927.02680310025007
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