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March 26, 1927


Author Affiliations

From the medical clinic of the Peter Bent Brigham Hospital.

JAMA. 1927;88(13):985-987. doi:10.1001/jama.1927.02680390013004

This paper reports observations on the value of the blood diazo test in the prognosis of nephritis. Our attention was drawn to the possible clinical importance of this test by the papers of Andrewes1 and Hewitt2 of England, Becher3 of Germany and Rabinowitch4 of Canada. We cannot find any mention of it in American medical literature.

Andrewes, while studying the van den Bergh method for estimating bile pigments in serum, noted a characteristic pink reaction which developed in the serum of uremic patients when the protein-free filtrate was treated with Ehrlich's diazo reagent and made alkaline. This reaction was not obtained in other conditions than uremia, and appeared to be a simple clinical test of considerable diagnostic importance.

A few months later, Becher also reported a positive diazo reaction in certain nephritic cases. From previous studies, he had come to believe that the clinical picture of