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December 16, 1933


JAMA. 1933;101(25):1972. doi:10.1001/jama.1933.02740500052017

Hemolytic streptococci of human origin are apparently unique among the pathogenic bacteria of clinical interest, since they invariably possess the property of rapidly dissolving or liquefying human fibrin. This lytic property may account in part for the ability of the streptococcus to invade tissues. A simple method for the determination of the fibrinolytic action of bacteria or of bacterial filtrates was developed by Tillett and Garner1 of Johns Hopkins University School of Medicine. The technic consists in the addition of 0.5 cc. of a broth culture of the micro-organism to be tested to 1 cc. of 20 per cent oxalated human plasma. Coagulation is induced by the addition of 0.25 cc. of 0.25 per cent calcium chloride solution and the tubes are immediately placed in a water bath at 37.5 C. Slight fibrinolysis is shown by a softening and release of the clot from the sides of the tube.