It is now fourteen years since the original announcement by Minot and Murphy1 of the effectiveness of liver therapy in pernicious anemia. During this period several groups of workers have attacked the problem of the isolation of the hemopoietically active material. It is fair to say that up to the present no such chemically pure and therapeutically active material has yet been isolated.2 On the other hand, numerous advances toward this goal have been made, and it is our intent in this communication to summarize the present state of progress.
The history of the isolation from natural sources of biologically active materials, in all probability, reveals no instance replete with the difficulties attending the isolation of the antianemic principle of liver. This unique character of the liver principle is mainly consequent on the fact that for all other biologically active materials there exist satisfactory methods for the assay
JACOBSON BM, SUBBAROW Y. STUDIES OF THE PRINCIPLE IN LIVER EFFECTIVE IN PERNICIOUS ANEMIA: VI. RECENT ADVANCES IN THE PURIFICATION OF ACTIVE SUBSTANCES. JAMA. 1941;116(5):367–374. doi:10.1001/jama.1941.02820050011003
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