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September 13, 1941


Author Affiliations

Bryn Mawr, Pa.

JAMA. 1941;117(11):954. doi:10.1001/jama.1941.02820370050026

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To the Editor:—  May I add a comment to the interesting and important question of examination of tissue with the electron microscope?It is important always to have in mind that any change in the water content away from the normal of cells or tissues results in a change in their internal structure. In the study reported by Seymour and Benmosche (The Journal, May 31, p. 2489) the cells examined had been in distilled water, a medium not iso-osmotic with the normal fluid surrounding of any body cells. Immersion in distilled water would result in an imbibition swelling, which would alter the physical cell structure considerably from its living condition. This is equally true of most technical methods, whether they involve drying, boiling or freezing or any means that alters normal water relations. Until some method can be devised for examining cells or tissues in an accurately iso-osmotic medium, interpretation

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