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Article
September 11, 1943

Current Comment

JAMA. 1943;123(2):96-97. doi:10.1001/jama.1943.02840370028011
Abstract

DEHYDRATED NERVE GRAFTS  To avoid sacrificing a "minor" nerve for repair of a more vital one, numerous zoologists have tried grafts of preserved or fixed nerves, such as petrolatum stored grafts or grafts fixed in alcohol or solution of formaldehyde. None of these have proved satisfactory. Since these failures were presumably due to denaturation of the stored nerves, Weiss and his colleagues1 of the department of zoology, University of Chicago, have developed a nondenaturing preservative method. Nerves dissected aseptically were dropped into isopentane which was immersed in liquid nitrogen at —195 C., where the nerves froze instantaneously. The frozen nerves were then dehydrated over phosphorus pentoxide in a high vacuum at —40 C. and the resulting dried nerves were stored in sealed aseptic containers. Before using, the stored nerves were rehydrated usually by immersion in isotonic solution of three chlorides in vacuo at room temperature, where they resumed their

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