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Article
September 5, 1931

MATERIAL FROM LIVER ACTIVE IN PERNICIOUS ANEMIA: FURTHER OBSERVATIONS

JAMA. 1931;97(10):685-687. doi:10.1001/jama.1931.02730100009004
Abstract

Since the demonstration by Minot and Murphy1 of the efficacy of a diet rich in liver in the treatment of pernicious anemia, efforts have been made to isolate the substances in liver responsible for these therapeutic effects.

Various properties of the antianemic material have been determined by several workers2 and last year amorphous barium derivatives and crystalline quinine derivatives of nitrogenous acids were obtained from liver which were followed by positive responses when given intravenously or subcutaneously to patients suffering from pernicious anemia. Doses of from 200 to 250 mg. were followed by maximal reticulocyte responses and a definite increase of both red blood cells and hemoglobin, the effects of a single dose lasting from two to three weeks.

From the barium derivatives after hydrolysis two amino-acids have been isolated, β-hydroxyglutamic acid and γ-hydroxyproline, and a quinine salt has been obtained the properties of which suggest that it

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