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August 31, 1929


Author Affiliations


From the Biological Division of the Medical Clinic, Johns Hopkins University and Hospital.

JAMA. 1929;93(9):692-693. doi:10.1001/jama.1929.02710090032010

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Glands removed in the department of orthopedics of the Johns Hopkins Hospital by Dr. William S. Baer from patients with infectious arthritis have been sent immediately to the biologic division of the medical clinic for culture. This note is made on account of numerous requests for the method and to record the positive observations.

METHOD  By previous arrangement the necessary mediums and instruments are ready for the preparation of the cultures of the gland immediately after removal. If other work prevents immediate culture, the glands are moistened with sterile isotonic salt solution, previously warmed to 37 C., and placed in the incubator. The glands are rinsed twice with warm isotonic salt solution and ground in the sterile tissue press shown in the accompanying illustration. Then 1 cc. of beef-infusionbroth ph 7.4 is added with a pipet while the ground gland remains in the press and suspension accomplished by blowing back

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