In the search for epidemic strains of hemolytic streptococci, earlier investigations were related to the isolation of mucoid strains of hemolytic streptococci. It was pointed out that mucoid encapsulated streptococci have epidemiologic significance.1 Milk borne epidemics of sore throat and scarlet fever have yielded consistently mucoid forms of beta hemolytic streptococci (Streptococcus epidemicus) if proper mediums and differential methods have been employed.2
To identify these mucoid strains readily a culture medium of ascites fluid added to fresh infusion blood agar has been described.3 As ascites fluid is not always available, the present study was directed to find a simple medium that could be used routinely for the rapid isolation of mucoid strains for grouping and typing.
Various mediums used in army laboratories were tried. Dehydrated brain heart infusion as prepared by Difco with 1.75 per cent agar furnished a satisfactory base for blood agar. Fresh moist plates
Pilot I. THE RAPID IDENTIFICATION OF MUCOID HEMOLYTIC STREPTOCOCCI OF POSSIBLE EPIDEMIC ORIGIN. JAMA. 1944;125(15):1037. doi:10.1001/jama.1944.72850330003008a
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