The methylene blue, test described by Franke1 as a method for the detection of bilirubin in urine was used by Stokes and Neefe2 in an outbreak of infectious (epidemic) hepatitis with results which indicated its possible value in the early diagnosis of the disease. In further investigating a modified methylene blue test suggested by Franke and first employed by Fellinger and Menkes,3 Neefe4 found the modified test to be of value in determining the presence of biliary pigments in the urine in the preicteric stage of the disease and occasionally before a significant increase in the concentration of total serum bilirubin was found. Because of its simplicity the modified test, although somewhat less sensitive than the Harrison spot test, was employed during the course of an investigation of infectious (epidemic) hepatitis in the Mediterranean theater of operations from November 1944 to February 1945. The present report
GELLIS SS, STOKES J. THE METHYLENE BLUE TEST IN INFECTIOUS (EPIDEMIC) HEPATITIS. JAMA. 1945;128(11):782–783. doi:10.1001/jama.1945.02860280008003
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