The production of antibodies in lymph nodes was first demonstrated by McMaster and Hudack1 by grinding lymph nodes of 20 to 30 mice with sand and determining antibody titers in the extract. The observations of McMaster and Hudack were confirmed by Ehrich and Harris2 by comparing antibody titers in the pad of the hind foot of the rabbit (which was the site of injection of antigen), the lymph contained in the peripheral lymph vessels, the popliteal lymph node (the only node regional to the site of injection), the efferent lymph and the blood serum.
As to the cells involved in antibody formation, Ehrich and Harris first showed that the cellular response in the lymph node during antibody production was chiefly a lymphocytic one, and the simultaneous rise of cells in the efferent lymph from about 18,000 per cubic millimeter of lymph to 60,000 or more was almost entirely
THE CELLULAR SOURCES OF ANTIBODIES. JAMA. 1945;128(17):1232. doi:10.1001/jama.1945.02860340038012
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