In April 1944 the preliminary report by the members of the Commission on Influenza on the prophylactic value of vaccination against influenza appeared in The Journal.1 The virus for the preparation of the vaccine was obtained from allantoic fluid of embryonated hen's eggs inoculated forty-eight hours earlier. The virus was concentrated ten times in isotonic solution of sodium chloride following adsorption to and elution from the embryonic erythrocytes. The infectious capacity was inactivated by solution of formaldehyde in a concentration of 1:5,000. Phenyl mercuric nitrate 1:100,000 or borate 1:50,000 was then added for bacteriostatic purposes. Each cubic centimeter of the vaccine was made up of 0.5 cc. representing type A virus recovered from 5 cc. of allantoic fluid and 0.5 cc. representing the type B virus recovered from 5 cc. of allantoic fluid. The type A component represented equal parts of the PR8 strain and of the Weiss strain,
VACCINATION AGAINST INFLUENZA. JAMA. 1945;129(9):619–620. doi:10.1001/jama.1945.02860430035012
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