It has been known for some time that tuberculin is extremely heat resistant and that it adheres to glass and other materials after they have been washed and sterilized. Attention has been called to this fact by such workers as Zieler,1 Smith,2 Parish and O'Brien 3 and Schick,4 and similar facts have been demonstrated for other allergens by Rackemann and Simon.5 Krause,6 in recalling experiments performed some years ago, has recently called attention to the precautions necessary to secure trustworthy results in immunologic experiments with extracts of tubercle bacilli. We have ourselves repeatedly secured positive reactions in tuberculin-hypersensitive persons when only physiologic solution of sodium chloride (0.85 per cent) was used in syringes previously employed for tuberculin, although these syringes had been washed and boiled and autoclaved. However, it has become increasingly evident to our two groups that insufficient attention is paid to this factor,
NELSON WE, SEIBERT FB, LONG ER. TECHNICAL FACTORS AFFECTING THE TUBERCULIN TEST. JAMA. 1937;108(26):2179–2181. doi:10.1001/jama.1937.02780260007002
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