In reviewing the extensive literature on tetanus, the reader will have forcibly brought home to him the absence of much specific work on the isolation of tetanus bacilli from street dust. As far as could be ascertained, practically no worker has concentrated on this particular phase of the distribution of tetanus spores except Nicolaier1 in 1884 and Bossano2 in 1889, who examined samples of both street dust and soil for the presence of tetanus spores. Their method, however, consisted of the injection of mixed material or impure liquid cultures into laboratory animals. Subsequent workers have taken for granted the existence of spores of tetanus in street dust, as is evidenced by the repeated statements to this effect in the literature in general. So deep rooted is the idea that tetanus spores exist in street dust that, apart from the use of tetanus antitoxin both as a prophylactic and
GILLES EC. THE ISOLATION OF TETANUS BACILLI FROM STREET DUST: ITS BEARING ON SURGICAL PRACTICE. JAMA. 1937;109(7):484–486. doi:10.1001/jama.1937.02780330012004
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