[Skip to Content]
[Skip to Content Landing]
Other Articles
December 11, 1937

DIAGNOSTIC METHODS IN UNDULANT FEVER (BRUCELLOSIS): WITH RESULTS OF A SURVEY OF 8,124 PERSONS

Author Affiliations

ELOISE, MICH.; EAST LANSING, MICH.

From the Departments of Pathology of Eloise Hospital, Eloise and Wayne University College of Medicine, Detroit, and the Department of Bacteriology, Michigan State College, East Lansing.

JAMA. 1937;109(24):1971-1974. doi:10.1001/jama.1937.02780500027009
Abstract

The purposes of this paper are (a) to describe briefly the performance and interpretation of the laboratory methods which at present are believed to be most useful in the diagnosis of undulant fever (brucellosis) and (b) to report some of the results of a survey of the incidence of brucellosis in a large county hospital.

LABORATORY METHODS  The laboratory methods which are regarded as most useful are (1) the brucellergin intradermal test, (2) the opsonic (opsonocytophagic) test (3) the rapid agglutination test and (4) culture (isolation) of Brucella.1

  1. The brucellergintest is performed by injecting 0.1 cc. of a suspensoid of nucleoprotein (1:2,000) isolated from Brucella cells intradermally on the forearm and is read after forty-eight hours (fig. 1).

  2. The brucella opsonic test is performed by incubating a mixture of a live forty-eight hour culture of Brucella abortus and the patient's citrated blood in a water bath at

×